Jervell and Lange-Nielsen syndrome (JLNS) is one of the most severe life-threatening cardiac arrhythmias. This disease cell model belongs to long-QT syndrome type 1, mutation (E160fs, also called c.478-2A>T) occur in the KCNQ1 gene. Generating pluripotent stem cells from fibroblasts, then differentiate into cardiac myocytes. This disease cell model provides a platform to explore disease mechanisms in human genetic cardiac disorders.