5-FAM
The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.
| Catalog Number | CDX-C0014-M025 |
| Alternative Name(s) | 5-Carboxyfluorescein |
| Research Area | Biochemicals, Immunology |
| Molecular Formula | C21H12O7 |
| CAS# | 76823-03-5 |
| Purity | >99% |
| Inchi | InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)28-18-9-12(23)3-6-15(18)19(14)13-4-1-10(20(24)25)7-16(13)21(26)27/h1-9,22H,(H,24,25)(H,26,27) |
| Inchi Key | VPMWLNJETPKSSC-UHFFFAOYSA-N |
| SMILES | OC(=O)C1=CC=C(C(=C1)C(O)=O)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12 |
| Size | 25 mg |
| Supplier Page | http://www.adipogen.com/cdx-c0014/5-fam.html |