TMB dihydrochloride

TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (lambdamax: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.

Price Not Available 1 g TMB dihydrochloride Supplier Page
Catalog Number CDX-T0151-G001
Alternative Name(s) 3,3′,5,5′-Tetramethylbenzidine dihydrochloride; TMBZ
Research Area Biochemicals, Immunology
Molecular Formula C16H20N2 . 2HCl
CAS# 64285-73-0
Purity >98%
Inchi InChI=1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
Inchi Key NYNRGZULARUZCC-UHFFFAOYSA-N
SMILES Cl[H].Cl[H].CC1=CC(=CC(C)=C1N)C1=CC(C)=C(N)C(C)=C1
Size 1 g
Supplier Page http://www.adipogen.com/cdx-t0151/tmbz-hcl.html