ZnAF-1F and ZnAF-2F do not fluoresce in the presence of other biologically important cations such as Ca2+ and Mg2+, and are insensitive to change of pH. The complexes with Zn2+ of previously developed ZnAFs, ZnAF-1, and ZnAF-2 decrease in fluorescence intensity below pH 7.0 owing to protonation of the phenolic hydroxyl group of fluorescein, whose pKa value is 6.2. On the other hand, the Zn2+ complexes of ZnAF-1F and ZnAF-2F emit stable fluorescence under neutral and slightly acidic conditions because the pKa values are shifted to 4.9 by substitution of electron-withdrawing fluorine at the ortho position of the phenolic hydroxyl group. Spectral data: lambdaex 492nm; lambdaem 517nm in PBS.

Price Not Available 5 mg ZnAF-1F Supplier Page
Catalog Number CDX-Z0009-M005
Alternative Name(s) 5-{2-[Bis(2-pyridylmethyl)amino]ethylamino}-2′,7′-difluorofluorescein
Research Area Biochemicals, Immunology
Molecular Formula C34H26F2N4O5
CAS# 443302-08-7
Purity >90%
Inchi InChI=1S/C34H26F2N4O5/c35-27-14-25-31(16-29(27)41)44-32-17-30(42)28(36)15-26(32)34(25)24-8-7-20(13-23(24)33(43)45-34)39-11-12-40(18-21-5-1-3-9-37-21)19-22-6-2-4-10-38-22/h1-10,13-17,39,41-42H,11-12,18-19H2
Size 5 mg
Supplier Page http://www.adipogen.com/cdx-z0009/znaf-1f.html