6-Thioguanosine (6-thio-G) has applications in ribozyme and siRNA research, as well as in RNA-protein interactions. The removal of the silyl protecting group without interfering with the sulfur is critical. This is removed1 cleanly by triethylamine trihydrofluoride in DMSO but t-butylammonium fluoride (TBAF) leads to degradation of the thio-nucleotide analogue and should not be used.

This reagent can be used to create the 3′-phosphate by adding it as the first addition to the support. After ammonia deprotection the oligo will have the 3′-phosphate attached to the 2nd base added during synthesis. Both the support base and the CPR are cleaved. The DMT-group is removed during the ammonium hydroxide deprotection and […]

5-Carboxy-dC is a remarkable compound, utilized in the research of dire ailments of cancer and viral infections. Its resolute nature unveiling potent antitumor and antiviral attributes, ingeniously directed towards selective cellular pathways.