For 5′-attachment, we have found 5′-Cholesterol-CE Phosphoramidite (LK2170) to offer specific advantages in oligo synthesis. Since the cholesterol is attached directly to aminohexanol, it is not susceptible to 1,2-diol elimination as observed in some other products. Lack of a trityl group simplifies purification; some cholesterol products must be used in trityl-on mode (to prevent 1,2-diol […]