A label that can be used for preparing maleimide and iodoacetamide derivatives of 3-phenylcoumarin fluorophore. These derivatives can be self-quenching until they react with thiol groups and regain their strong fluorescence.
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A label that can be used for preparing maleimide and iodoacetamide derivatives of 3-phenylcoumarin fluorophore. These derivatives can be self-quenching until they react with thiol groups and regain their strong fluorescence.
More Information Supplier Page A label that can be used for preparing maleimide and iodoacetamide derivatives of 3-phenylcoumarin fluorophore. These derivatives can be self-quenching until they react with thiol groups and regain their strong fluorescence.
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A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It […]
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A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It […]
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The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at […]
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The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at […]
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Fluorescent probe with high quantum efficiency, exhibiting single exponential decay kinetics. The radiative fluorescence lifetime is almost constant and independent on molecular surroundings allowing this probe to be utilized in a range of microsurroundings with exciting radiation at a constant wavelength (in the blue spectral range). Its fluorescence emission demonstrates a large solvatochromic shift which […]
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Fluorescent probe with high quantum efficiency, exhibiting single exponential decay kinetics. The radiative fluorescence lifetime is almost constant and independent on molecular surroundings allowing this probe to be utilized in a range of microsurroundings with exciting radiation at a constant wavelength (in the blue spectral range). Its fluorescence emission demonstrates a large solvatochromic shift which […]
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Nonfluorescent N-substituted maleimides react selectively with thiol compounds to form fluorescent derivatives. Fluorescent intensity of the dimethoxyphthalimidyl group is higher than that of the nonsubsituted phthalimidyl group. The analytical sensitivity of N-[4-(5,6-Dimethoxy-N-phthalimidyl)phenyl]maleimide (DPM) is about 3-times higher than that of N-[4-(2-phthalimidyl)phenyl]maleimide (PPM). DPM can be used as a precolumn labeling reagent in HPLC. DPM was […]
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