HiLyte Fluor 750 acid, SE is the longest-wavelength amine-reactive HiLyte Fluor dye currently available. It has excitation and emission spectrum peak wavelengths of approximately 754 nm/ 778 nm. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte Fluor 647, HiLyte Fluor 680 or allophycocyanin (APC), facilitating multicolor analysis. The HiLyte Fluor 750-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications.