Scoulerine ((-)-Scoulerine), an isoquinoline alkaloid, is a potent antimitotic compound. Scoulerine is also an inhibitor of BACE1 (?-site amyloid precursor protein cleaving enzyme 1). Scoulerine inhibits proliferation, arrests cell cycle, and induces apoptosis in cancer cells.In Vitro: Scoulerine ((-)-Scoulerine) inhibits mini-panel of human leukemic cells, MOLT-4 (WT), Jurkat (TP53 mutated), Raji (TP53 mutated), HL-60 (TP53 null), U-937 (TP53 mutated), and HEL 92.1.7 (wild-type), with IC50s ranging from 2.7?μM to 6.5?μM.
Scoulerine (2.5-20?μM; 24 hours) decreases proliferation of Jurkat and MOLT-4 cells.
Scoulerine (2.5-20?μM; 24 hours) induces MOLT-4 and Jurkat cells apoptosis.
Scoulerine induces G2 or M cell cycle arrest.
Scoulerine (2.5-5?μM; 24?hours) shows an upregulation of p53 protein in p53 wild-type MOLT-4 cells.
Scoulerine (2.5-5?μM; 24-48 hours) activates caspase-3/7, -8 and -9 in a dose-dependent manner.
Scoulerine (5-10 μM; 24 hours) disrupts microtubule structure of A549 lung carcinoma cells