8-Prenylnaringenin is a phytoestrogen isolated and purified from the herbs of Humulus lupulus with the highest estrogenic activity, shows slight activity in this assay but significant activity by immunostaining, improves mitochondrial function, whereas a high dose of XN (5 µM) worsens the functionality of this organelle, and has more potent effects on promoting osteoblastic bone formation and inhibiting osteoclastic bone resorption by ERα instead of ERβ than the two classic phytoestrogens: genistein and daidzein.