Pyrimethanil is an?anilinopyrimidine?and broad-spectrum?contact fungicide for the control of Botrytis spp. on a wide variety of crops. Pyrimethanil inhibits the biosynthesis of methionine and other amino acids in?Botrytis cinerea. Pyrimethanil can be used for the research of fungal diseases prevention on fruit, vegetable and ornamental plants with mold infection.In Vitro: Pyrimethanil is a strobilurin fungicide belonging to the anilinopyrimidine class. Pyrimethanil (5 and 50?μg/L) elicits a range of toxic responses, has the potential to induce histological alterations in tissues of the Italian tree frog?H.?intermedia after a long term exposure to two environmentally relevant concentrations of Pyrimethanil[2].Pyrimethanil decreases polygalacturonase, cellulase, proteinase and laccase activities in the medium of three day‐old cultures. The pyrimethanil results in 50% reduction (IC50) in total enzyme activities are approximately 0.25 μM for polygalacturonase, cellulase and proteinase, and approximately 1.0 μM for laccase.