Pipemidic acid (Acido pipemidico) is a pyridopyrimidine antibiotic derivative of piromidic acid with activity against gram-negative bacteria including Pseudomonas aeruginosa and some gram-positive bacteria. Pipemidic acid is an inhibitor of DNA gyrase that is a bacterial enzyme which catalyzes the ATP-dependent negative supercoiling of DNA. It can be used for the research of intestinal, urinary, and biliary tract infections.