β-Elemene ((-)-β-Elemene; Levo-β-elemene) is isolated from natural plant Curcuma aromatica with an antitumor activity. β-Elemene can induce cell apoptosis.In Vitro: β-Elemene (0-200 μg/ml; 24 hours) shows IC50 values of 72.8 μg/ml; 47.4 μg/ml; 61.5 μg/ml; 3.661 μg/ml; 68 μg/ml; 72.12 μg/ml; 37.894 μg/ml and 37.703 μg/ml for SV-HUC-1, T24, 5637, TCCSUP, J82,UMUC-3,RT4, and SW780 cells, respectively.β-Elemene (0-75 μg/ml; 24 hours) decreases cell number from 50 μg/ml and is notably decreased at 75 μg/ml, ?induces dose-dependent G1-phase arrest in T24cells and significantly reduces the percentage of cells in the S-phase?. β-Elemene (50 μg/ml; 12 hours) downregulates the expression levels of p-STAT3 and the cell cycle-related proteins cyclin D1, CDK4 and CDK6, and upregulates p21 and?p27expression in T24 cells?. β-Elemene (50 μg/ml; 24 hours) enhances cisplatin-induced apoptosis by activating the ROS-AMPK signaling pathway.