Ninhydrin can be used as a chromogenic analytical probe for the quantification of amino acids and proteins.IC50 & Target: IC50: chromogenic analytical probeIn Vitro: Ninhydrin is used in amino acid analysis of proteins. Except?proline, Most amino acids can be?hydrolyzed?and react with ninhydrin. The amino acids are then quantified colorimetrically after separation by?chromatography.Ninhydrin reacts with primary and secondary amines producing a blue or purple reaction product: diketohydrindylidene-diketohydrindamine.