ZnAF-1F is a potent fluorophore for with an Kd value of 2.2 nM. ZnAF-1F can be used as fluorescent probes for Zn2+ in cells. ZnAF-1F shows λ excitation of 489 nm and λ emission of 514 nm[2].In Vitro: ZnAF-1F (1 μM) shows an Kon value of 3.5*106 M-1s-1, and an Koff value of 7.7*10-3 s-1 in 100mM HEPES buffer.
ZnAF-1F is a Zn sensors in neutral and slightly acidic conditions[2].