5′-O-DMT-N2-DMF-2′-deoxyguanosine 3′-CE phosphoramidite, an indispensable constituent in the biomedical sector, finds extensive application in the synthesis of oligonucleotides for diagnostics and research studies. Its pivotal role encompasses the progress of nucleic acid-centered therapies and drug exploration, with a primary focus on combatting a wide range of diseases, encompassing cancer, genetic disorders, and viral infections.