Desmosterol is the immediate unsaturated precursor of cholesterol and the final biosynthetic intermediate in the Bloch pathway and a key activator of liver X receptors(LXR) while inhibiting SREBP to regulate lipid homeostasis. Its reduction promotes mitochondrial ROS production in macrophages and activates the NLRP3 inflammasome exhibiting potential for research in inflammation, metabolism, and cardiovascular diseases.