RB-3, a PRC1 inhibitor, binds to RING1B-BMI1f, with a Kd of 2.8 μM.IC50 & Target:Kd: 2.8 μM (RING1B-BMI1f).In Vitro: RB-3 decreases the global level of H2A ubiquitination and induces differentiation in leukemia cell lines and primary acute myeloid leukemia (AML) samples.
RB-3 inhibits H2A ubiquitination with an IC50 of 1.6 μM.
RB-3 inhibits PRC1 and disrupts the interaction with nucleosomes.
RB-3 reduces colony formation in a mixed lineage leukemia (MLL)-eleven nineteen leukemia (ENL) model.