PKUMDL-LC-101-D04(GPX4-Activator-1d4) is a potent allosteric activator of Glutathione peroxidase 4 (GPX4) effectively suppressing ferroptosis and inflammation. It enhances GPX4 activity by 150% at 20 µM in a cell-free assay and 61 µM in cell extracts, also providing protection against cholesterol hydroperoxide (ChOOH)-induced damage.