NLRP3-IN-NBC6 is a potent, selective NLRP3 inflammasome inhibitor (IC50= 574 nM) that acts independently of Ca2+. NLRP3-IN-NBC6 inhibits Nigericin (HY-127019)-induced inflammasome activation in THP-1 cells and Imiquimod (HY-B0180)-induced IL-1β release from LPS-primed bone marrow-derived macrophages (BMDMs).In Vitro: In LPS-primed primary BMDMs, NLRP3-IN-NBC6 (10 μM; 15 minutes) inhibits Imiquimod-induced IL-1β release. NLRP3-IN-NBC6 completely inhibits NLRP3-dependent IL-1β release in neutrophils (neutrophils were isolated from WT and NLRP3 KO murine bone marrow and primed with LPS followed by Nigericin treatment in the presence of 10 μM NLRP3-IN-NBC6).