NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed. Storage: protect from light.In Vitro:NSP-SA-NHS marking
(1) 1mg BSA was dissolved in 0.3 mL PBS (solution A), and 0.2 mg NSP-SA-NHS was dissolved in 40 μL DMF (solution B).
(2) Add solution B to solution A, mix at 18°C, 130 rpm, and dark for 12 h.
(3) The mixture was dialyzed with 500 mL of distilled water at 4°C for 2 d, during which distilled water was changed twice (solution C).
(4) Slowly add solution B to solution C, mix at 18°C, 130 rpm, and dark for 24 h, and then dialysis with distilled water for 48 h. During this period, change distilled water four times.
(5) Dilute the coupling compound with distilled water to a suitable concentration, and analyze it with an ultraviolet spectrophotometer.