Sp-cAMPS sodium salt, a cAMP analog, is potent activator of cAMP-dependent PKA I and PKA II. Sp-cAMPS sodium salt is also a potent, competitive phosphodiesterase (PDE3A) inhibitor with a Ki of 47.6 μM. Sp-cAMPS sodium salt binds the PDE10 GAF domain with an EC50 of 40 μM[2].In Vitro:Treatment of hepatocytes with Sp-cAMPS sodium salt, the stimulatory diastereomer of adenosine cyclic 3′,5′-phosphorothioate, mimics the response seen with glucagon. The glucagon-stimulated increases in the level of Ca2+ can be mimicked by Sp-cAMPS sodium salt.In Vivo:In chronic alcohol consumption (CAC) mice, direct infusion of the Sp-cAMPS (1 μg/μL) sodium salt into the prefrontal cortex significantly improves or impairs, respectively, working memory performance in withdrawn and water animals.