5-Formyl-dC, an indispensable reagent in the biomedicine field, occupies a pivotal position owing to its unparalleled characteristics. Its significance lies in facilitating the synthesis of tailored nucleic acids, thereby facilitating in-depth exploration of various research domains. Remarkably, this compound imparts unprecedented opportunities for the discovery of novel therapeutic agents and diagnostic instruments targeting a broad spectrum of ailments spanning cancer and hereditary anomalies.