Propargylcholine bromide is a choline analogue containing terminal propargyl that can be incorporated into all classes of Choline-containing phospholipids such as phosphatidylcholine and sphingomyelin, labeling Choline-containing phospholipids. Propargylcholine bromide-labeled phospholipid molecules can be visualized in cells with high sensitivity and spatial resolution. Propargylcholine bromide can be used as a molecular tool to study the biochemical and metabolic processes of Choline-containing phospholipids in cells[2].In Vitro:Propargylcholine bromide (10, 50 μM; 24 h) incorporates into NIH 3T3 cells and shows strong staining in a concentration-dependent manner (fixed and stained with Alexa568-azide).In Vivo:Propargylcholine bromide (P-Cho; 3.5-4.0 mg/kg; i.p; single daily for 6 days) effectively incorporates into newly synthesized myelin within 1 week of dosing and sustained presence beyond 6 weeks in rhesus monkey[2].