NLRP3/AIM2-IN-3 (compound 59) is a potent inhibitor with species-specific effects on NLRP3 and AIM2 inflammasome-mediated pyroptosis. It demonstrates potent inhibition of pyroptosis in THP-1 macrophages stimulated with LPS/nigericin, with an IC50 of 0.077 μM. It also disrupts the interaction between NLRP3 or AIM2 and the adaptor protein ASC, effectively preventing ASC oligomerization and blocking inflammasome assembly.