Gypenoside L, is a saponin from Gynostemma pentaphyllum, increases SA-β-galactosidase activity, enhances senescence-associated cytokine production, inhibits proliferation and LPS-induced nitric oxide (NO) production, induces S-phase arrest, and activates p21, p27, p38, ERK MAPK, and NF-κB pathways to promote senescence.