Paromomycin is an aminoglycoside antibiotic that is effective against Gram-negative and Gram-positive bacteria (MICs = 0.08-3.9 μg/ml). It also has antiprotozoal properties and decreases the parasite burden of mice infected with the leishmanial strain L. tropica when administered at doses of 50 mg/kg and 15 mg/kg, through intramuscular or intralesional routes, respectively. Paromomycin binds to the tRNA decoding A site of the 16S ribosomal RNA in bacteria, inducing a conformational change that disrupts translation and inhibits protein synthesis.