TRIM is a potent nitric oxide synthase inhibitor. TRIM inhibits mouse cerebellar nNOS and rat lung iNOS in vitro with IC50 values of 28.2 and 27.0 μM, respectively. Antidepressant- and anxiolytic-like effects[2].In Vitro: TRIM is a relatively weak inhibitor of eNOS (IC50, 1057.5 μM).