VU0420373 is a potent heme sensor system (HssRS) activator with an EC50 of 10.7 μM and a pEC50 of 4.97. VU0420373 induces heme biosynthesis, and is toxic to fermenting S. aureus.IC50 & Target: EC50: 10.7 μM (HssRS)
pEC50: 4.97 (HssRS)In Vitro: VU0420373 (compound 2e) has an IC50 of >60 μM,for Newman aerobic and Newman anaerobic, respectively.
VU0420373 (40 μM; 2, 4, 6, 8 hours) is able to preadapt S. aureus to a toxic concentration of heme (20 μM).