Rhodamine-N3 chloride is an azide-rhodamine fluorescent dye that can be used to label biomolecules containing alkyne groups[2].In Vitro: The cell lysates or cells are treated with probes (0.001-10 μM, 1 hr), and then reacted with an Rhodamine-N3 (N3-Rh) reporter tag under copper-catalyzed azide-alkyne cycloaddition (CuACC or click chemistry) conditions and probe-labeled proteins visualized by SDS-PAGE and in-gel fluorescence scanning[2].