Poly-D-lysine hydrobromide (MW 30000-70000) is a synthetic polymeric substrate and is one of the most widely used substrate in neural cell culture. Poly-D-lysine hydrobromide (MW 30000-70000) removes proteinase K-resistant PrP from prion-infected SN56 neuroblastoma cells without affecting PrPC[2].In Vitro: Poly-D-lysine is immunogenic in rabbits only when injected at low doses.
The approximate concentration of Poly-D-lysine (PDL) needed to lower the amount of PrPres by 50% corresponds to ~0.8 μg/mL or 50 nM[2].