NBD-14189 is a potent HIV-1 entry antagonist with an IC50?of 89 nM against the HIV-1HXB2?pseudovirus. NBD-14189 binds to HIV-1 gp120 and shows potent antiviral activity (EC50<200 nM)[2].In Vitro: NBD-14189 has anti-HIV-1 activity (IC50=0.089 μM) and cytotoxicity (CC50=21.9 μM) in single-cycle (TZM-bl cells) assays. NBD-14189 has anti-HIV-1 activity (IC50=0.18 μM) and cytotoxicity (CC50=22.1 μM) in multi-cycle (MT-2 cells) assays.
NBD-14189?(0-50 μM) prevents HIV-1 mediated cell-cell fusion with an IC50?of 9.4 μM in indicator cells TZM-bl cells were cocultured with Env- and Tat-expressing HL2/3 cells.
NBD-14189?shows weak or no inhibition of the hERG current, with an IC50?of 3.0 μM, respectively.