2-Acetamidofluorene is a potent carcinogenan. 2-Acetamidofluorene is can be used fot induction of hepatocellular carcinoma (HCC) and multiple primary tumours[2].In Vivo: 2-Acetamidofluorene-induced HCC rat liver tissues has hepatic cell degeneration, oval cell proliferation, and inflammatory cell infiltration.