(2S,3S)-tert-Butyl 2-((tert-Butoxycarbonyl)amino)-6-chloro-3-hydroxyhexanoate is an intermediate for the synthesis of 3S-Hydroxy-N6,N6,N6-trimethyl-L-lysine Chloride (H977645), which is a metabolite in the biosynthesis of carnitine and acts as a biomarker in its analysis. Carnitine is a compound that enables intracellular transport of fatty acids.