TFEB activator 1 is an orally effective, mTOR-independent activator of TFEB. It significantly promotes the nuclear translocation of Flag-TFEB with an EC50 of 2167 nM and enhances autophagy without inhibiting the mTOR pathway and has the potential for neurodegenerative diseases treatment. It also shows cytotoxic effect on HeLa, K562, MCF-​7 and MDA-​MB-​231 cancer cell lines.