Bacterial, fungal, viral, and other microbial RNA-Seq can provide unbiased strand-specific information on novel and known transcripts and help annotate and quantify microbial transcripts. Microbial isoform sequencing (Iso-Seq) is an ideal method to analyze microbial transcriptomes and gene fusion events, and characterize gene isoforms and alternative splicing events. Our microbial iso-seq service can provide full-length reads with no assembly needed, spanning entire transcript isoforms from the polyA-tail to the 5′ end using PacBio Single Molecular and Real-Time (SMRT) technologies.