7-Amino-4-methylcoumarin is a natural product isolated and purified from an endophytic Xylaria fungal species and exhibited strong antibacterial and antifungal activities in vitro against Aspergillus, Candida, Escherichia, Staphylococcus, Salmonella and Vibrio species, among others. It is also a reagent employed in the preparation of fluorogenic AMC-based substrates for the detection of proteolytic enzyme activity. AMC has been utilized as a fluorescent probe to analyze glycoproteins’ monosaccharides and N-linked oligosaccharides via chromatography and has been used as a laser dye.